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KMID : 0370219760200030125
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Yakhak Hoeji
1976 Volume.20 No. 3 p.125 ~ p.129
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Gas-liquid Chromatographic Analysis of Some Tropane Alkaloids
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백남호/Paik NH
임용빈/박만기/In YB/Park MK
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Abstract
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The aim of this investigation was to develop a quantitative gas-liquid chromatographic method of analysis for hyoscyamine and scopolamine, and to apply this method to the analysis of preparations. The trimethylsilyl(TMS) derivatives of the alkaloids were found to be far superior to the nonsilylating compounds in chromatograms. Bis(trimethylsilyl)acetamide(BSA) was evaluated and found to be a good reagent for silylation of the alkaloids. The optimum derivatization conditions were heating the alkaloids in a closed tube at 70o for 30 min with a 150 molar excess of BSA to the alkaloids. Calibration curves for the two alkaloids were found to be linear over a sample weight range of 1~9mcg of each alkaloid. The standard deviations were 1.1% for hyoscyamine and 1.5% for scopolamine. The minimum detectable amount using the hydrogen flame ionization detector was determined to be 2 X 10-11 moles of each alkaloid injected.
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